Characterization of mitochondrial Lace1 ATPase (LB168)

Isolation and Antigenic Characterization of Corn Mitochondrial F(1)-ATPase.

Corn mitochondrial F(1)-ATPase was purified from submitochondrial particles by chloroform extraction. Enzyme stored in ammonium sulfate at 4 degrees C was substantially activated by ATP, while enzyme stored at -70 degrees C in 25% glycerol was not. Enzyme in glycerol remained fully active (8-9 micromoles P(i) released per minute per milligram), while the ammonium sulfate preparations steadily l...

Biogenesis of mitochondrial ATPase.

Mitochondrial ATPase Complex

A stable, oligomycin-sensitive, ATPase preparation can be isolated from rat liver mitochondria by refinement of the deoxycholate-solubilization procedure (Soper, J. W., and Pedersen, P. L. (1976) Biochemistry 15, 2682). NADM dehydrogenase, succinic dehydrogenase, and cytochrome oxidase activities cannot be detected by enzymatic assay. Spectral measurements reveal that the content of individual ...

LACE1 interacts with p53 and mediates its mitochondrial translocation and apoptosis

p53 is a major cellular tumor suppressor that in addition to its nuclear, transcription-dependent activity is also known to function extranuclearly. Cellular stressors such as reactive oxygen species can promote translocation of p53 into mitochondria where it acts to protect mitochondrial genome or trigger cell death via transcription-independent manner. Here we report that the mammalian homolo...

Influence of aurovertin on mitochondrial ATPase activity.

Investigations have been made of the kinetic effects of the antibiotic aurovertin on the ATPase and ITPase activity of isolated rat liver mitochondrial ATPase. Unusual patterns of inhibition, decreasing slope, and increasing y-intercept values of double reciprocal plots, were observed with Mg-ATP as the substrate under various conditions. Under specified conditions, aurovertin stimulated hydrol...